Register Login

PLASTIC RECONSTRUCTIVE AND REGENERATIVE SURGERY

Original article

Blood Transfusion - 1 2020 (January-February)

Cigarette smoking and antioxidant defences in packed red blood cells prior to storage

Authors

Renata E. Boehm , Sabrina N. Do Nascimento , Carolina R. Cohen , Solange Bandiera , Rianne R. Pulcinelli , Almeri M. Balsan , Nuryan S. Fao , Caroline Peruzzi , Solange C. Garcia , Leo Sekine , Tor G.H. Onsten , Rosane Gomez

Key words: blood donation, cotinine, oxidative stress, tobacco, vitamins
Doi: 10.2450/2019.0166-19
Publication Date: 2019-11-20

Abstract

Background. Red blood cells from smoking donors can have more lesions from oxidative stress, decreasing the benefits of blood transfusion. We aimed to explore the effect of cigarette smoking on the oxidative status of packed red blood cells (PRBCs) prior to storage.
Materials and methods. We compared serum vitamin C, plasmatic malondialdehyde (MDA), and non-protein thiol groups (GSH) levels in PRBCs, as well glutathione peroxidase (GPx) and glutathione s-transferase (GST) activity in PRBCs from smoking (n=36) and non-smoking (n=36) donors. We also correlated urinary cotinine levels with these parameters.
Results. Cigarette smoking was associated with decreased serum levels of vitamin C and GPx, and increased GST activity in PRBCs. We found negative correlations between cotinine, GPx activity and vitamin C levels, and a positive correlation between cotinine and GST activity.
Discussion. Cigarette smoking changed antioxidant defences of PRBCs prior to storage and these parameters are correlated with cotinine levels. Increased RBC antioxidants such as GST may reflect an exposure to oxidants during erythropoiesis. Because of the inability of mature RBCs to resynthesise antioxidants, PRBCs from smokers may have higher risk of storage lesions than those from non-smoker donors.

References

Downloads

PDF
Authors

Renata E. Boehm Post-graduate Progamme in Pharmacology and Therapeutics, Universidade Federal do Rio Grande do Sul (UFRGS); Haemotherapy Unit of the Hospital de Clínicas de Porto Alegre (HCPA)

Sabrina N. Do Nascimento Toxicology Laboratory, Department of Analyses, (LATOX), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil

Carolina R. Cohen Haemotherapy Unit of the Hospital de Clínicas de Porto Alegre (HCPA)

Solange Bandiera Post-graduate Progamme in Pharmacology and Therapeutics, Universidade Federal do Rio Grande do Sul (UFRGS)

Rianne R. Pulcinelli Post-graduate Progamme in Pharmacology and Therapeutics, Universidade Federal do Rio Grande do Sul (UFRGS)

Almeri M. Balsan Haemotherapy Unit of the Hospital de Clínicas de Porto Alegre (HCPA)

Nuryan S. Fao Toxicology Laboratory, Department of Analyses, (LATOX), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil

Caroline Peruzzi Toxicology Laboratory, Department of Analyses, (LATOX), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil

Solange C. Garcia Toxicology Laboratory, Department of Analyses, (LATOX), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil

Leo Sekine Haemotherapy Unit of the Hospital de Clínicas de Porto Alegre (HCPA)

Tor G.H. Onsten Haemotherapy Unit of the Hospital de Clínicas de Porto Alegre (HCPA)

Rosane Gomez Post-graduate Progamme in Pharmacology and Therapeutics, Universidade Federal do Rio Grande do Sul (UFRGS)

  • Abstract viewed - 585 times
  • PDF downloaded - 154 times